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Human IgG1-kappa antibody to SARS CoV-2 S1 RBD (clone 27B12)

 

Figure 1. Coomassie-stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike RBD, clone 27B12. 4-12% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 27B12 (-DTT). Lane 2. Size Marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 27B12 (+DTT). Lane 4. Size Marker

       

Figure 2. HPLC analytical SEC for final product.

       

Figure 2. HPLC analytical SEC for final product.

         

Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Spike S1 protein.

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Catalogue #

R1-157-100

Name

Human IgG1-kappa antibody to SARS CoV-2 S1 RBD (clone 27B12)

Target

SARS-CoV-2 virus

Target Description

SARS-CoV-2 virus (PBMCs isolated from patients recovered from SARS-CoV-2 infection, antibodies discovered by using HybriFree Technology)

Clonality

Human monoclonal

Clone

27B12

Class

hIgG1

Reactivity

SARS-CoV-2 Spike RBD

Dissociation constant (KD)

< 1.0 x 10-12 M (measured against SARS-CoV-2 Trimeric Spike protein)

2.02 x 10-8 M (measured against SARS-CoV-2 Spike S1 protein)

Application

ELISA

ELISA:

0,02-0,04 ng/ml

Purification

Protein A affinity chromatography following desalting

Buffer

PBS pH 7.4

Shipping

Shipped at ambient temperature.

Storage

Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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