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Figure 1. Coomassie-stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike RBD, clone 27B12. 4-12% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 27B12 (-DTT). Lane 2. Size Marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 27B12 (+DTT). Lane 4. Size Marker
Figure 2. HPLC analytical SEC for final product.
Figure 2. HPLC analytical SEC for final product.
Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Spike S1 protein.
Catalogue #
R1-157-100
Name
Human IgG1-kappa antibody to SARS CoV-2 S1 RBD (clone 27B12)
Target
SARS-CoV-2 virus
Target Description
SARS-CoV-2 virus (PBMCs isolated from patients recovered from SARS-CoV-2 infection, antibodies discovered by using HybriFree Technology)
Clonality
Human monoclonal
Clone
27B12
Class
hIgG1
Reactivity
SARS-CoV-2 Spike RBD
Dissociation constant (KD)
< 1.0 x 10-12Â M (measured against SARS-CoV-2 Trimeric Spike protein)
2.02 x 10-8Â M (measured against SARS-CoV-2 Spike S1 protein)
Application
ELISA
ELISA:
0,02-0,04 ng/ml
Purification
Protein A affinity chromatography following desalting
Buffer
PBS pH 7.4
Shipping
Shipped at ambient temperature.
Storage
Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
© 2023 Icosagen
