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Figure 1. Simply Blue Safe Stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Nucleocapsid protein, clone 82C3. 4-15% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Nucleocapsid protein, clone 82C3 (+DTT). Lane 2. Size marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Nucleocapsid protein, clone 82C3 (-DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet RED96e analysis, different concentrations of antibody (200, 100,50,25 and 12.5 nM) was loaded on sensor for capture of Nucleocapsid protein.
Catalogue #
R1-179-100
Name
Human IgG1-kappa antibody to SARS CoV-2 NP (clone 82C3)
Target
Nucleocapsid protein SARS-CoV-2
Target Description
Recombinant SARS-CoV-2 Nucleocapsid protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line
Clonality
Human monoclonal
Clone
82C3
Class
hIgG1
Reactivity
SARS-CoV-2 Nucleocapsid protein
Dissociation constant (KD)
< 1.0 x 10-12 M
Application
ELISA
Kd:
<1.0 x 10-12 M
ELISA:
indirect ELISA 0,005-0,01 ng/ml
Purification
Protein A affinity chromatography following desalting
Buffer
PBS pH 7.4
Shipping
Shipped at ambient temperature.
Storage
Store at +4 °C. Avoid multiple freeze-thaw cycles.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
© 2024 Icosagen
