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Figure 1. Coomassie-stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Nucleocapsid protein, clone 43E5. 10% gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Nucleocapsid protein, clone 43E5 (+DTT). Lane 2. Size marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Nucleocapsid protein, clone 43E5 (-DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Nucleocapsid protein in different concentrations (200, 100, 50 and 25 nM).
Catalogue #
R1-167-100
Name
Human IgG1-lambda antibody to SARS CoV-2 NP (clone 43E5)
Target
Nucleocapsid protein SARS-CoV-2
Target Description
Recombinant SARS-CoV-2 Nucleocapsid protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line
Clonality
Human monoclonal
Clone
43E5
Class
hIgG1
Reactivity
SARS-CoV-2 Nucleocapsid protein
Dissociation constant (KD)
2.23 x 10-9Â M
Application
ELISA
Kd:
2.23 x 10-9Â M
ELISA:
indirect ELISA 0,02-0,04 ng/ml
Purification
Protein A affinity chromatography following gel filtration
Buffer
PBS pH 7.4
Shipping
Shipped at ambient temperature.
Storage
Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
© 2023 Icosagen
