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Human IgG2 mAb to cardiac troponin complex, clone 5F8

 

Figure 1. Coomassie stained SDS-PAGE analysis of monoclonal antibody to cardiac troponin I, clone 5F8. 4-15% gradient gel is used for analysis. Lane 1. Monoclonal antibody to cardiac troponin I, 5F8 (-DTT). Lane 2. Size marker. Lane 3. Monoclonal antibody to cardiac troponin I, 5F8 (+DTT).

       

Figure 2. Analytical SEC of final product.

       

Figure 3. Octet RED96e analysis, antibody was loaded on sensor for capture of human cardiac troponin complex.

       

Figure 4. Octet RED96e analysis, biotinylated human cardiac troponin complex was loaded on sensor for capture of the antibody.

                                         

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Quantity:

€ 2098

Catalogue #

R1-126-100

Name

Human IgG2 mAb to cardiac troponin complex, clone 5F8

Target

Recombinant human cardiac troponin complex

Target Description

Recombinant human cardiac troponin complex protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line

Clonality

Human monoclonal

Clone

5F8

Class

hIgG2

Reactivity

human cardiac troponin I

Dissociation constant (KD)

1.60 x 10-10 M (Troponin complex binding from solution)

1 x 10-12 M (Troponin complex binding from streptavidin surface)

Application

ELISA

Purification

MabSelect affinity chromatography following gel filtration

Buffer

50 mM Na-citrate, pH 6.0, 0.9 % NaCI, 0.095 % NaN3 (SPTN)

Unit Size

100 µg

Related Products

Monoclonal antibodies to human cardiac troponin I. For more information visit www.www.icosagen.com/antibodies

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

Background

Troponin I is a part of the troponin complex. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it, myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle (Wikipedia)

Limitations

This product is for research use only.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

Our locations

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San Francisco, CA 94108
+1 415 548 6625

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+372 737 7070
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