Human IgG2 mAb to cardiac troponin complex, clone 5F8

Catalogue #

R1-126-100

Name

Human IgG2 mAb to cardiac troponin complex, clone 5F8

Target

Recombinant human cardiac troponin complex

Target Description

Recombinant human cardiac troponin complex protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line

Clonality

Human monoclonal

Clone

5F8

Class

hIgG2

Reactivity

human cardiac troponin I

Dissociation constant (K_D)

1.60 x 10^-10 M (Troponin complex binding from solution)

1 x 10^-12 M (Troponin complex binding from streptavidin surface)

Application

ELISA, CLIA, WB

Purification

MabSelect affinity chromatography following gel filtration

Buffer

50 mM Na-citrate, pH 6.0, 0.9 % NaCI, 0.095 % NaN3 (SPTN)

Related Products

Monoclonal antibodies to human cardiac troponin I. For more information visit www.www.icosagen.com/antibodies

Shipping

This product is shipped in non-frozen liquid form on blue ice.

Storage

Stored refrigerated at +2…+8°C for short term. Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.

Background

Troponin I is a part of the troponin complex. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it, myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle (Wikipedia)

Limitations

This product is for research use only.