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MANF, human recombinant

 

Figure 1. SDS-PAGE analysis of human MANF visualized by PageBlue protein staining solution (Fermentas). Line 1. Protein size marker (PageRuler Prestained protein ladder, Fermentas); Line 2. 10 µg of MANF.

 

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Catalogue #

P-101-100

Name

MANF, human recombinant

Synonyms

Mesencephalic astrocyte - derived neurotrophic factor – MANF; ARMET; ARP

Uniprot ID

P55145

Source

Human

MW

Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids

Host

CHO-based cell line (expressed by QMCF Technology)

Purification

Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media

Concentration

1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard

Buffer

PBS pH 7.4, concentration

Endotoxine

Less than 1EU/mg of protein as determined by LAL method

Bioproperties

Protein is biologically active. Suitable for cell culture and in vivo testing. Human recombinant MANF has been tested in rat 6-OHDA model of Parkinson's disease and shown neuroprotection and neurorestoration. MANF has also shown neuroprotection in animal models of stroke

QC

SDS-PAGE and Western-Blot analysis; Mass-spectroscopy: Purified human MANF is characterized as homogenous material by mass-spectroscopy

Related Products

Polyclonal and monoclonal antibodies against human MANF For more information please visit: www.icosagen.com/products/?antibodies

Shipping

Shipped on dry ice

Storage

Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles

Background

MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta

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This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com
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