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Figure 1. Simply Blue Safe Stained SDS-PAGE analysis of mAb to cardiac troponin I, clone 8B2. 4-15% gradient gel is used for analysis. Lane 1. Monoclonal antibody to cardiac troponin I, 8B2 (-DTT) Lane 2. Size marker. Lane 3. Monoclonal antibody to cardiac troponin I, 8B2 (+DTT)
Figure 2. Analytical SEC of final product.
Figure 3. Octet RED96e analysis, antibody was loaded on sensor for capture of human cardiac troponin complex.
Figure 4. Octet RED96e analysis, biotinylated human cardiac troponin complex was loaded on sensor for capture of the antibody.Catalogue #
R1-128-100
Name
Human IgG1 mAb to cardiac troponin I, clone 8B2
Target
Human cardiac troponin I
Target Description
Recombinant human cardiac troponin complex protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line
Clonality
Human monoclonal
Clone
8B2
Class
hIgG1
Reactivity
Human cardiac troponin I
Dissociation constant (KD)
6.18 x 10-09 M (troponin complex binding from solution)
8.81 x 10-11 M1 (troponin complex binding from streptavidin surface)
Application
ELISA, CLIA
Purification
Protein A affinity chromatography following gel filtration
Concentration
1 mg/ml
Buffer
PBS, pH 7.4
QC
Simply Blue Safe stained SDS-PAGE, analytical SEC, Octet binding
Shipping
Shipped on blue ice.
Storage
Refrigerated at +2…+8°C for short term. Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
© 2024 Icosagen
