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Figure 1. Immunofluorescence detection of human RET expressed in U2OS cells. RET was visualized using Chicken polyclonal antibody to human RET (dilution 1:500) and Goat anti-chicken IgY - H&L DyLight 550 (Abcam, ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for RET specific signal and blue for nuclei
Figure 2. Western-Blot detection of human RET expressed in CHO cells. Line 1. hRET-containing CHOEBNALT85 cell culture supernatant. Line 2. Negative control - CHOEBNALT85 cell culture supernatant. 5 μl of supernatant were loaded per line. Primary antibody dilution 1:5000 was used. Rb pAb to Chk IgY ab6753, was used as secondary antibody.Catalogue #
306-100
Name
Chicken pAb to human RET
Target
human RET
Target Description
Recombinant His-tagged extracellular fragment of human RET protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. The extracellular fragment of hRET was expressed and secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant
Alternative Names
Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12
Proto-oncogene c-Ret
Uniprot ID
P07949
Clonality
Polyclonal
Class
IgY
Reactivity
Human RET
Application
ELISA, WB, IF
Protocol
ELISA:
8-12,5 ng/ml
WB:
0,1-0,2 µg/ml
IF:
2-4 µg/ml
Purification
RET affinity purified
Buffer
Concentrated ammonium sulphate in PBS pH 7.4
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Background
The RET proto-oncogene is a receptor tyrosine kinase for members of the glial cell line-derived neurotrophic factor family of extracellular signalling molecules
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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