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Chicken pAb to human RET

  Figure 1. Immunofluorescence detection of human RET expressed in U2OS cells. RET was visualized using Chicken polyclonal antibody to human RET (dilution 1:500) and Goat anti-chicken IgY - H&L DyLight 550 (Abcam, ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for RET specific signal and blue for nuclei   Figure 2. Western-Blot detection of human RET expressed in CHO cells. Line 1. hRET-containing CHOEBNALT85 cell culture supernatant. Line 2. Negative control - CHOEBNALT85 cell culture supernatant. 5 μl of supernatant were loaded per line. Primary antibody dilution 1:5000 was used. Rb pAb to Chk IgY ab6753, was used as secondary antibody.

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Quantity:

€ 2098

Catalogue #

306-100

Name

Chicken pAb to human RET

Target

human RET

Target Description

Recombinant His-tagged extracellular fragment of human RET protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. The extracellular fragment of hRET was expressed and secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant

Alternative Names

Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12
Proto-oncogene c-Ret

Uniprot ID

P07949

Clonality

Polyclonal

Class

IgY

Reactivity

Human RET

Application

ELISA, WB, IF

Protocol

Polyclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

8-12,5 ng/ml

WB:

0,1-0,2 µg/ml

IF:

2-4 µg/ml

Purification

RET affinity purified

Buffer

Concentrated ammonium sulphate in PBS pH 7.4

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use

Background

The RET proto-oncogene is a receptor tyrosine kinase for members of the glial cell line-derived neurotrophic factor family of extracellular signalling molecules

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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