Search from website

Chicken pAb to human GFRa-4

Figure 1. IF detection of human GFRα-4 expressed in U2OS cells. GFRα-4 was visualized using Chicken polyclonal antibody to human GFRα-4 (dilution 1:2000) and Goat anti-chicken IgY - H&L DyLight 550 (Abcam, ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRα-4 specific signal and blue for nuclei. Figure 2. Western-Blot detection of human GFRα-4, using Chicken polyclonal antibody to human GFRα-4. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. hGFRα-4. Primary antibody dilution 1:5000 was used. Line 2. Prestained protein ladder (Naxo 8003). Rabbit anti-chicken antibody, HRP conjugate (903-100, Icosagen) was used as secondary antibody.

Select volume:

Quantity:

€ 2098

Catalogue #

308-100

Name

Chicken pAb to human GFRa-4

Target

human GFRa-4

Target Description

Recombinant His-tagged human GFRa-4 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRa-4, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant

Alternative Names

GDNF receptor alpha-4, Persephin receptor

Uniprot ID

Q9GZZ7

Clonality

Polyclonal

Class

IgY

Reactivity

Human GFRa-4, doesn’t cross-react with hGFRa-1, hGFRa-2, hGFRa-3

Application

ELISA, WB, IF

Protocol

Polyclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

3-6,7 µg/ml

WB:

0,1-0,2 µg/ml

IF:

0,3-1 µg/ml

Purification

GFRa-4 affinity purified

Buffer

PBS pH 7.4

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.

Background

GFRα-4 belongs to the GDNF receptor family. It is a glycosyl-phosphatidylinositol (GPI)-linked cell surface receptor for persephin, and mediates activation of the RET tyrosine kinase receptor

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com
error:
Shopping Cart