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Figure 1. IF detection of human GFRα-4 expressed in U2OS cells. GFRα-4 was visualized using Chicken polyclonal antibody to human GFRα-4 (dilution 1:2000) and Goat anti-chicken IgY - H&L DyLight 550 (Abcam, ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRα-4 specific signal and blue for nuclei. 
Figure 2. Western-Blot detection of human GFRα-4, using Chicken polyclonal antibody to human GFRα-4. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. hGFRα-4. Primary antibody dilution 1:5000 was used. Line 2. Prestained protein ladder (Naxo 8003). Rabbit anti-chicken antibody, HRP conjugate (903-100, Icosagen) was used as secondary antibody.Catalogue #
308-100
Name
Chicken pAb to human GFRa-4
Target
human GFRa-4
Target Description
Recombinant His-tagged human GFRa-4 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRa-4, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant
Alternative Names
GDNF receptor alpha-4, Persephin receptor
Uniprot ID
Q9GZZ7
Clonality
Polyclonal
Class
IgY
Reactivity
Human GFRa-4, doesn’t cross-react with hGFRa-1, hGFRa-2, hGFRa-3
Application
ELISA, WB, IF
Protocol
ELISA:
3-6,7 µg/ml
WB:
0,1-0,2 µg/ml
IF:
0,3-1 µg/ml
Purification
GFRa-4 affinity purified
Buffer
PBS pH 7.4, 0.1% sodium azide
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.
Background
GFRα-4 belongs to the GDNF receptor family. It is a glycosyl-phosphatidylinositol (GPI)-linked cell surface receptor for persephin, and mediates activation of the RET tyrosine kinase receptor
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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