Publication: New 𝗜𝗻𝘀𝗶𝗴𝗵𝘁𝘀 𝗶𝗻𝘁𝗼 𝗗𝗡𝗔 𝗥𝗲𝗽𝗹𝗶𝗰𝗮𝘁𝗶𝗼𝗻 – 𝗔 𝗖𝗼𝗻𝘀𝗲𝗿𝘃𝗲𝗱 𝗣𝗵𝗼𝘀𝗽𝗵𝗼𝗿𝘆𝗹𝗮𝘁𝗶𝗼𝗻 𝗠𝗲𝗰𝗵𝗮𝗻𝗶𝘀𝗺 Controls 𝗥𝗲𝗴𝘂𝗹𝗮𝘁𝗶𝗻𝗴 𝗖𝗠𝗚 𝗛𝗲𝗹𝗶𝗰𝗮𝘀𝗲 could Lead to New Therapeutics

A conserved phosphorylation mechanism for regulating the interaction between the CMG replicative helicase and its forked DNA substrate

Exciting new results from research group led by Ivar Ilves reveals a 𝗵𝗶𝗴𝗵𝗹𝘆 𝗰𝗼𝗻𝘀𝗲𝗿𝘃𝗲𝗱 𝗽𝗵𝗼𝘀𝗽𝗵𝗼𝗿𝘆𝗹𝗮𝘁𝗶𝗼𝗻 𝗺𝗲𝗰𝗵𝗮𝗻𝗶𝘀𝗺 that regulates the interaction between the 𝗖𝗠𝗚 𝗵𝗲𝗹𝗶𝗰𝗮𝘀𝗲 and its forked DNA substrate. Our very own QC Manager (GMP and GMP Process Development) Nele Tamberg and Key Account and Technology Officer for our Transient protein production - Lauri Peil were co-authors for the article published in the Journal of Biological Chemistry, their study deepens our understanding of how cells control 𝗴𝗲𝗻𝗼𝗺𝗶𝗰 𝗗𝗡𝗔 𝗿𝗲𝗽𝗹𝗶𝗰𝗮𝘁𝗶𝗼𝗻, a fundamental process essential for life.

𝗪𝗵𝘆 𝗱𝗼𝗲𝘀 𝘁𝗵𝗶𝘀 𝗺𝗮𝘁𝘁𝗲𝗿?

🔹 𝗔 𝗨𝗻𝗶𝘃𝗲𝗿𝘀𝗮𝗹 𝗖𝗼𝗻𝘁𝗿𝗼𝗹 𝗦𝘆𝘀𝘁𝗲𝗺: The study finds that a phosphorylation site in the MCM3 subunit of CMG is conserved from yeast to humans, suggesting a shared regulatory mechanism across eukaryotes.
🔹 𝗜𝗺𝗽𝗹𝗶𝗰𝗮𝘁𝗶𝗼𝗻𝘀 𝗳𝗼𝗿 𝗖𝗮𝗻𝗰𝗲𝗿 & 𝗚𝗲𝗻𝗲𝘁𝗶𝗰 𝗗𝗶𝘀𝗼𝗿𝗱𝗲𝗿𝘀: Since errors in DNA replication are linked to cancer and genetic diseases, understanding this regulation could lead to new therapeutic strategies.
🔹 𝗣𝗼𝘁𝗲𝗻𝘁𝗶𝗮𝗹 𝗶𝗻 𝗕𝗶𝗼𝘁𝗲𝗰𝗵𝗻𝗼𝗹𝗼𝗴𝘆: The ability to fine-tune DNA replication could be useful in fields like synthetic biology and genomic medicine.

𝗞𝗲𝘆 𝗙𝗶𝗻𝗱𝗶𝗻𝗴𝘀 & 𝗔𝗱𝘃𝗮𝗻𝗰𝗲𝘀:

✅ Discovery of a conserved phosphorylation site in the MCM3 subunit of the CMG helicase.
✅ Proposed mechanism: Phosphorylation at this site controls how the helicase interacts with DNA, ensuring accurate and efficient replication.
✅ Potential role in stress response: This modification may help stabilize replication forks during cellular stress.

This research not only deepens our understanding of 𝗵𝗼𝘄 𝗗𝗡𝗔 𝗿𝗲𝗽𝗹𝗶𝗰𝗮𝘁𝗶𝗼𝗻 𝗶𝘀 𝗿𝗲𝗴𝘂𝗹𝗮𝘁𝗲𝗱 but also opens doors for future applications in cancer research, biotechnology, and disease modeling.

Kudos to the authors for this outstanding contribution!

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