Figure 1. Simply Blue stained SDS-PAGE analysis of SARS-CoV-2 Trimeric Spike Brazil P1 . 4-12% gradient gel is used for analysis. Lane 1. 1.3 µg SARS-CoV-2 Trimeric Spike Brazil P1 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 1.3 µg SARS-CoV-2 Trimeric Spike Brazil P1 (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet Red96e analysis of SARS-CoV-2 Trimeric Spike Brazil P1 binding to human ACE2 receptor.
SARS-CoV-2 Trimeric Spike Brazil P1 (gamma)
Protein contains amino acids 14-1211 and following mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F. Plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS). Also, two stabilizing prolines (PP) have been added to the S2 domain.
CHO-based cell line (expressed by QMCF Technology)
Purified by Ni-affinity chromatography and gel-filtration from serum-free CHO growth media, sterile filtrated
PBS pH 7.4
Measured by its binding ability to ACE2 protein by OCTET RED96 system.
SDS-PAGE, analytical SEC, Octet binding to ACE2 receptor
Shipped on dry ice.
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
This product is for research use only
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