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SARS-CoV-2 Spike S1 South Africa VOC 501.V2 (beta)

SARS-CoV-2 Spike S1 South Africa VOC 501.V2 (beta)  

Figure 1.  Coomassie-stained SDS-PAGE analysis of SARS-CoV-2 Spike S1 South Africa VOC 501.V2. 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg  SARS-CoV-2 Spike S1 South Africa VOC 501.V2 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 0.8 µg SARS-CoV-2 Spike S1 South Africa VOC 501.V2 (+DTT).

      SARS-CoV-2 Spike S1 South Africa VOC 501.V2 (beta)  

Figure 2. HPLC analytical SEC for final product.

        SARS-CoV-2 Spike S1 South Africa VOC 501.V2 (beta)  

Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.

          SARS-CoV-2 Spike S1 South Africa VOC 501.V2 (beta)  

Figure 4. Binding analysis of the SARS-CoV-2 Spike S1 South Africa VOC 501.V2. protein to hACE2 receptor. hACE2 -Fc protein was bound to Protein G sensor. 200 nanomols of SARS-CoV-2 Spike S1 protein was bound to the ACE2 receptor.

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Quantity:

€ 1650

Catalogue #

P-319-100

Name

SARS-CoV-2 Spike S1 South Africa VOC 501.V2 (beta)

Description

Protein contains amino acids 14-681, mutations L18F, D80A, D215G, LAL 242-244 del, R246I, K417N, E484K, N501Y, D614G, two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag.

MW

75.45 kDA

Host

CHO-based cell line (expressed by QMCF Technology)

Purification

Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.

Purity

>95%

Concentration

1 mg/ml

Buffer

PBS pH 7.4

Endotoxine

NA

Bioproperties

Measured by its binding ability to ACE2 protein by OCTET RED96 system.

QC

SDS-PAGE
NanoDrop A280, analytical SEC, Octet binding to ACE2 receptor

Shipping

Shipped on dry ice

Storage

Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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