Otsi
Otsi
Figure 1. SDS-PAGE analysis in reduced and non-reduced conditions. 1.3 µg of SARS-CoV-2 Spike RBD2 UK VOC 202012/01 protein were loaded per lane. Lane 1.SARS-CoV-2 Spike RBD2 UK VOC 202012/01 (reduced); Lane 2. Protein size marker (Smobio); Lane 3. SARS-CoV-2 Spike RBD2 UK VOC 202012/01 (non-reduced).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet Red96e analysis of SARS-CoV-2 Spike RBD2 UK VOC 202012/01 binding to human ACE2 receptor.
Catalogue #
P-311-100
Name
SARS-CoV-2 Spike RBD2 UK VOC 202012/01(alpha)
Description
Protein contains amino acids 319-541, mutation N501Y, plus two extra amino acids (YS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag.
MW
26.3 kDa
Host
CHO-based cell line (expressed by QMCF Technology)
Kd:
2.22E-09 (measured against ACE2 receptor)
Purification
Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
Purity
>95%
Concentration
1 mg/ml
Buffer
PBS pH 7.4
Endotoxine
NA
QC
SDS-PAGE
NanoDrop A280
analytical SEC, Octet binding to ACE2 receptor
Shipping
Shipped on dry ice
Storage
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
Background
Measured by its binding ability to ACE2 protein by OCTET RED96 system.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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