Otsi
Otsi
Figure 1. Simply Blue Safe stained SDS-PAGE analysis of SARS-CoV-2 Spike RBD2 B.1.621. 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg SARS-CoV-2 Spike RBD2 B.1.621 (-DTT). Lane 2. Protein marker (Smobio). Lane 3. 0.8 µg SARS-CoV-2 Spike RBD2 B.1.621 (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet Red96e analysis of SARS-CoV-2 Spike RBD2 B.1.621 binding to human ACE2 receptor.
Catalogue #
P-363-100
Name
SARS-CoV-2 Spike RBD2 B.1.621 (mu)
Description
Protein contains amino acids 319-537, mutations R346K, E484K, N501Y plus two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag.
Uniprot ID
P0DTC2
MW
26.3 kDa
Host
CHO-based cell line (expressed by QMCF Technology)
Purification
Purified by Ni-affinity chromatography and gel-filtration from serum-free CHO growth media, sterile filtrated
Concentration
1 mg/ml
Buffer
PBS pH 7.4
Endotoxine
NA
Bioproperties
Measured by its binding ability to ACE2 protein by OCTET RED96 system.
QC
SDS-PAGE, NanoDrop A280, Analytical SEC, Octet binding to ACE2
Shipping
Shipped on dry ice.
Storage
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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