Figure 1. SDSPAGE analysis in reduced and non-reduced conditions. 1.3 µg of S1 RBD2 protein were loaded per lane. Lane 1. RBD2 (-DTT); Lane 2. Protein size marker (Smobio); Lane 3. RBD2 (+DTT).
Figure 2. Octet Red96e analysis of SARS-CoV-2 S1 RBD2 binding to human ACE2 receptor.
Figure 3. HPLC analytical SEC for final product.
Figure 4. HPLC analytical SEC after 3 freeze-thaw cycles.
SARS-Cov-2 S1 RBD2
Protein contains amino acids 319-541, plus two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus.
CHO-based cell line (expressed by QMCF Technology)
Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
PBS pH 7.4
Measured by its binding ability to ACE2 protein by OCTET RED96 system.
NanoDrop A280, analytical SEC, Octet binding to ACE2 receptor
Shipped on dry ice
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
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