Mouse mAb to SIV-1 Nef (clone 17)

Catalogue #

A2-817-100

Name

Mouse mAb to SIV-1 Nef (clone 17)

Target

SIV-1 Nef

Target Description

Recombinant SIV-1 Mac251 Nef protein of (purified from E. coli)

Clonality

Mouse monoclonal

Clone

17

Class

mIgG1

Reactivity

Reacts in ELISA, WB, RIP, IHC with Nef of SIVmac251strain. (Piguet 1998, Khan 1998,Guillemin 2000). Reacts in IF with Nef of SIVmac239 strain and SIVmac1A11strain. (Khan 1998, Chan 2005).
Mapped to amino acids 71-80 (-PAEEREKLAY-, SIV-1)

Application

ELISA, WB, IHC, RIP

Protocol

WB:

1 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

References

Khan IH, Sawai ET, Antonio E, Weber CJ, Mandell CP, Montbriand P, Luciw PA. 1998.
Role of the SH3-Ligand Domain of Simian Immunodeficiency Virus Nef in Interaction with Nef-Associated Kinase and Simian AIDS in Rhesus Macaques. J. Virol. 72: 5820-30

Piguet V, Chen Y-L, Mangasarian A, Foti M, Carpentier J-L, Trono D. 1998.
Mechanism of Nef-induced CD4 endocytosis: Nef connects CD4 with the µ chain of adaptor complexes. EMBO, 17 (9): 2472-81.

Mandell, CP, Reyes RA, Cho K, Sawai ET, Fang AL, Schmidt KA, Luciw PA. 1999.
SIV/HIV Nef recombinant virus (SHIVnef) produces simian AIDS in rhesus macaques. Virology 265(2):235-51.

Sawai ET, Hamza MS, Ye M, Shaw KE, Luciw PA. 2000.
Pathogenic Conversion of Live Attenuated Simian Immunodeficiency Virus Vaccines Is Associated with Expression of Truncated Nef. Journal of Virology, 74(4): 2038-45.

Guillemin G, Croitoru J, Le-Grand R, Franck-Duchenne M, Dormont D, Boussin F D. 2000.
Simian immunodeficiency virus mac251 infection of astrocytes. J of Neurovirology 6(3): 173-86.

Chan KS, Verardi PH, Legrand FA, Yilma TD. 2005.
Nef from pathogenic simian immunodeficiency virus is a negative factor for vaccinia virus. PNAS 102(24): 8734-9.