Mouse mAb to HCV NS5B (clone 6B12)

Catalogue #

A2-420-100

Name

Mouse mAb to HCV NS5B (clone 6B12)

Target

HCV 1b NS5B

Target Description

Recombinant Hepatitis C Virus subtype 1b non-structural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)

Uniprot ID

Q9WMX2

Clonality

Mouse monoclonal

Clone

6B12

Class

mIgG1

Reactivity

Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 92-105 (TPPHSARSKFGYGA)

Application

ELISA, WB, IP

Protocol

ELISA:

1.2 µg/ml

WB:

in reduced conditions 1 µg/ml

IF:

-

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Background

Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.

References

Nikonov A, Juronen E, Ustav M 2008.
Functional characterization of fingers subdomain-specific monoclonal antibodies inhibiting the hepatitis C virus RNA-dependent RNA polymerase. J. Biol Chem. 283(35):24089-102.