Figure 1. IP was carried out with NS5B specific mAb 7G8 using the lysates of Huh7 cells harboring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (a-NS5B) and a-Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with NS5B specific mAb 10D6.
Figure 2. WB analysis of Huh-7 cell line expressing NS5B protein. Line 1. Huh-7 transfected with HCV replicon, Line 2. Huh-7 non-transfected cells.
Mouse mAb to HCV NS5B (clone 7G8)
HCV 1b NS5B
Recombinant Hepatitis C Virus subtype 1b non-structural protein 5B (NS5B)
Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 95-105(TPPHSARSKFGYGA)
ELISA, WB, IP
in reduced conditions 1 µg/ml
Protein G purification
PBS pH 7.4, with 0.1% sodium azide
This product is shipped in non-frozen liquid form in ambient conditions
Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.
Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.
Nikonov A, Juronen E, Ustav M 2008.
Functional characterization of fingers subdomain-specific monoclonal antibodies inhibiting the hepatitis C virus RNA-dependent RNA polymerase. J. Biol Chem. 283(35):24089-102.
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