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Figure 1. Western Blot testing of anti-BPV E2 monoclonal antibody (5E11).
A:Testing of purified recombinant BPV E2. Different amount of purified protein was loaded into the gel. Lane 1. 30 ng; Lane 2. 15 ng; Lane 3. 10 ng; Lane 4. 5 ng; Lane 5. 1 ng.
B: Testing of BPV E2 expressed in CHO cell line. Lane 1. Cell lysate of CHO cells. Lane 2. Cell lysate of CHO cells stably expressing BPV E2
Catalogue #
A1-100-100
Name
Mouse mAb to BPV type 1 E2-protein (clone 5E11)
Target
BPV type 1 E2
Target Description
Recombinant full length BPV type 1 E2 protein purified from E. coli
Uniprot ID
P03122
Clonality
Mouse monoclonal
Clone
5E11
Class
IgG
Reactivity
Bovine, BPV type 1 E2 protein amino acids 199-208 (SSTSSDFRDR)
Application
ELISA, WB, IF, IP
Protocol
ELISA:
0.02 µg/ml
WB:
0.2 - 1.2 µg/ml
IF:
0.4-24 µg/ml
Purification
Protein G purification
Buffer
PBS with 50% glycerol
Related Products
9 different monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: www.icosagen.com/products/?antibodies
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at -20 or -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Background
E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV
References
Koljak R, Järving I, Kurg R, Boeglin WE, Varvas K, Valmsen K, Ustav M, Brash AB, and Samel N. 2001.
The basis of prostaglandin synthesis in coral, J. Biol. Chem., 276: 7033-7040.
Nigul L, Olspert A, Meier M, Paves M, Talpsep T, and Truve E. 2004.
New Plant vectors for Protein Tagging with E2 Epitope, Plant Molecular Biology Reporter, 4: 399 – 407.
Mannan
AU, Boehm J, Sauter SM, Rauber A, Byrne PC, Neesen J, and Engel W. 2006. Spastin, the most commonly mutated protein in hereditary
spastic paraplegia interacts with reticulon 1 an endoplasmic reticulum protein.
Neurogenetics 7: 93-103.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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