Otsi
Otsi
Figure 1. Coomassie-stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike RBD, clone 60E10. 10% gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 60E10 (+DTT). Lane 2. Size marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 60E10 (-DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Spike S1 protein in different concentrations: 200 nM, 100 nM, 50 nM, 25 nM and 12.5 nM.
Catalogue #
R1-174-100
Name
Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 60E10)
Target
SARS-CoV-2 virus
Target Description
SARS-CoV-2 virus (PBMCs isolated from patients recovered from SARS-CoV-2 infection, antibodies discovered by using HybriFree Technology).
Clonality
Human monoclonal
Clone
60E10
Class
hIgG1
Reactivity
SARS-CoV-2 Spike RBD
Neutralizing properties: blocks S1 and ACE2 binding in Octet system; IC50 125 ng/ml measured in live virus neutralization assay
Dissociation constant (KD)
<1.0 x 10-12 M (measured against SARS-CoV-2 Trimeric Spike protein)
2.22 x 10-9 M (measured against SARS-CoV-2 Spike S1 protein)
Application
ELISA
ELISA:
0,02-0,04 ng/ml
Purification
Protein A affinity chromatography following gel filtration
Buffer
PBS pH 7.4
Shipping
Shipped at ambient temperature.
Storage
Store at +4 °C. Avoid multiple freeze-thaw cycles.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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