Otsi
Otsi
Figure 1. Simply Blue Safe stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike RBD, clone 49F9. 4-12% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 49F9 (-DTT). Lane 2. Size marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 49F9 (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Spike S1 protein in different concentrations (200, 100, 50, 25 and 12.5 nM).
Figure 5. Octet RED96e analysis, antibody was loaded on sensor for capture of Trimeric Spike protein in different concentrations (50, 25, 12.5, 6.25 and 3.13 nM).
Catalogue #
R1-169-100
Name
Human IgG1-lambda antibody to SARS-CoV-2 Spike S1 (clone 49F9)
Target
SARS-CoV-2 virus
Target Description
SARS-CoV-2 virus (PBMCs isolated from patients recovered from SARS-CoV-2 infection, antibodies discovered by using HybriFree Technology).
Clonality
Human monoclonal
Clone
49F9
Class
hIgG1
Reactivity
SARS-CoV-2 Spike RBD
Dissociation constant (KD)
1.15 x 10-11 M (measured against SARS-CoV-2 Trimeric Spike protein)
4.82 x 10-8 M (measured against SARS-CoV-2 Spike S1 protein)
Application
ELISA
ELISA:
0,01-0,02 ng/ml
Purification
Protein A affinity chromatography following gel filtration
Buffer
PBS pH 7.4
Shipping
Shipped at ambient temperature.
Storage
Store at +4 °C. Avoid multiple freeze-thaw cycles.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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