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Icosagen offers an in-house developed chemiluminescence immunoassay (CLIA) tailored for validating diagnostic antibodies. This assay is optimized for 384-well plates, ensuring maximum efficiency and throughput. With a streamlined workflow and minimized incubation steps, our method has significantly higher efficiency and sensitivity compared to traditional sandwich ELISA. We prioritize precision and consistency by implementing comprehensive Standard Operating Procedures (SOPs) for every stage of the project. Our team consists of highly experienced, and trained personnel who are proficient in performing CLIA assays, guaranteeing reliable and accurate results. 
Table 1. Critical parameters showing significantly higher efficiency for both, FGF-21 and IL-6, in CLIA over
ELISA.
Figure 1. One pair of antibodies against FGF-21 was tested in CLIA and ELISA for comparison of the two methods. Analyses were conducted in quadruplicates. The linear range of CLIA is about 2x larger than the one for ELISA. LoD and LoQ values reveal, respectively, about 10x and 5x higher sensitivity for CLIA over ELISA.
Figure 2. One pair of antibodies against IL-6 was tested in CLIA and ELISA for comparison of the two methods. Analyses were conducted in quadruplicates. The linear range of CLIA is about 2x larger than the one for ELISA. LoD and LoQ values reveal, respectively, about 10x and 5x higher sensitivity for CLIA over ELISA.
Antibody pairs against FGF-21, IL-6 and Galectin-3 show high sensitivity in CLIA over a larger linear range. Results are in line with the required sensitivity for diagnostic purposes. The obtained pairs are thus suitable for applications in diagnostics.
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