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Chemiluminescence immunoassay service

Icosagen offers an in-house developed chemiluminescence immunoassay (CLIA) tailored for validating diagnostic antibodies. This assay is optimized for 384-well plates, ensuring maximum efficiency and throughput. With a streamlined workflow and minimized incubation steps, our method has significantly higher efficiency and sensitivity compared to traditional sandwich ELISA. 
We prioritize precision and consistency by implementing comprehensive Standard Operating Procedures (SOPs) for every stage of the project. Our team consists of highly experienced, and trained personnel who are proficient in performing CLIA assays, guaranteeing reliable and accurate results.
 
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Why choose Icosagen as your CLIA service provider?

FAST
TURNAROUND
TIME

FAST TURNAROUND TIME

On average, with unbiotinylated detection antibodies, results in 3 working days.

CALCULATION OF
LoD & LoQ or
EC50

CALCULATION OF LoD & LoQ or EC50

Calculation of limit of detection (LoD) and limit of quantification (LoQ) for evaluating and comparing antibody pairs. Analysis of single antibody binding - EC50.

DOSE-RESPONSE
& STANDARD
CURVES

DOSE-RESPONSE CURVES

We include dose-response curves from indirect CLIA, using statistical software for analyzing (EC50), graphing, and presenting scientific data.

DETAILED
REPORTS

DETAILED REPORTS WITH READY TO USE GRAPHICS

We provide a comprehensive overview of the project, including experimental description, raw data, data analysis, standard curves or dose dependent curves, and conclusions with ready to use graphics.

TRAINED &
EXPERIENCED
PERSONNEL

TRAINED & EXPERIENCED PERSONNEL

Highly experienced and trained personnel in conducting CLIA assays.

ANTIBODY PAIR
EVALUATION

DOSE-RESPONSE CURVES

We offer antibody pair determination and evaluation to find most suitable pairs.

Case study with FGF-21 and IL-6: CLIA shows greater sensitivity (lower LoD and LoQ), and wider linear range than ELISA

Table 1. Critical parameters showing significantly higher efficiency for both, FGF-21 and IL-6, in CLIA over
ELISA.
Chemiluminescence immunoassay service
Chemiluminescence immunoassay service

Figure 1. One pair of antibodies against FGF-21 was tested in CLIA and ELISA for comparison of the two methods. Analyses were conducted in quadruplicates. The linear range of CLIA is about 2x larger than the one for ELISA. LoD and LoQ values reveal, respectively, about 10x and 5x higher sensitivity for CLIA over ELISA.

Chemiluminescence immunoassay service
Figure 2. One pair of antibodies against IL-6 was tested in CLIA and ELISA for comparison of the two methods. Analyses were conducted in quadruplicates. The linear range of CLIA is about 2x larger than the one for ELISA. LoD and LoQ values reveal, respectively, about 10x and 5x higher sensitivity for CLIA over ELISA.

Report example

Antibody pairs against FGF-21, IL-6 and Galectin-3 show high sensitivity in CLIA over a larger linear range. Results are in line with the required sensitivity for diagnostic purposes. The obtained pairs are thus suitable for applications in diagnostics.
Chemiluminescence immunoassay service
Chemiluminescence immunoassay service
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Let's discuss CLIA service!

Chemiluminescence immunoassay service
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