Expression of viral envelope or capside proteins results self-assembly of virus-like particles (VLP-s). VLP-s could be produced in different cell systems, including mammalian, insect, yeast and plant cells. One good possibility to produce membrane proteins in functional and correct conformational manner is expression of these proteins in composition of virus like particles (VLP-s). VLP-s could be used for different approaches e.g. vaccine development, investigation of receptor functions and also for purification of different membrane proteins.
Custom VLP Production Services include
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Cloning |
Expression analysis |
Expression optimization (optional) |
Scaled-up expression and purification |
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Codon optimization and synthesis of cDNA (optional) Cloning of cDNA into QMCF VLP expression plasmids |
Expression in suspension QMCF/293 cells in small scale Primary detection of protein and VLP expression Generation of expression cell banks |
Selection of expression vectors, optimization of growth media and conditions, cell lines Detection of protein and VLP expression Generation of expression cell banks |
Expanding the culture volume Detection of protein and VLP expression Purification of the product by ultracentrifugation or precipitation and gel-filtration
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3-4 weeks gene synthesis; 1 week cloning |
2-3 days |
Min 4-5 weeks |
Min 3-4 weeks |
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Price starts from EUR 2000 for cloning and expression analysis |
Inquire |
Price starts from EUR 2500 | |
