PROTEIN PROdUCTION in mammalian cells
Icosagen Cell Factory has established a mammalian protein production system, where the gene of interest is expressed from a plasmid that stably replicates and maintains itself in mammalian cells.
This new protein expression system is called QMCF Technology and it enables to perfom quick and efficient:
• production of recombinant proteins and antibodies generating productivities up to 1.0 g/L for early stage and preclinical research and for development of biologics
• expression of virus-like particles (VLPs) for vaccine development or productin of membrane proteins
QMCF Technology consists of specially designed vectors and modified mammalian cells, which are based on CHO or HEK293 cells. Using QMCF Technology a cDNA of a target molecule is cloned into QMCF expression vector and transfected by electroporation into QMCF cells, which are grown in serum-free suspension. A special mechanism ensures high copy number of QMCF plasmids and succesful propagation into dividing daughter cells. Compared to transient expression systems, where the production lasts only for few days, QMCF technology allows to extend the protein production cycle to 2-3 weeks. Thus the protein production yields achieved by QMCF system exceed significantly yields of transient expression. Freezing and thawing of transfected QMCF cells allow generation of cell banks and makes further up‐scale quick and cost‐effective.
Read more about QMCF Technology here.
