Smart Taq Thermostable DNA Polymerase

Cat No	Y2-102-005	Y2-102-025
Qty	500 U	2500 U
Source	Recombinant E.coli strain
Concentration	5 U/µl
Storage	- 20 oC

Enzyme properties

• Purified from an recombinant E.coli strain with cloned gene encoding Thermus aquaticus DNA polymerase.
• Taq DNA polymerase has 5’ -> 3’ DNA synthesis activity.

Quality data 

• Activity and stability tested at 20, 30 and 40 cycles of PCR reactions at 95°C.
• Tested for the absence of human DNA contamination by PCR with Alu-specific primers.

Unit definition
One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C. Storage Buffer
20 mM Tris-HCl (pH 8.0), 1mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5%, Nonidet P40, 0.5% Tween 20, 50% glycerol.

Smart Taq Thermostable DNA Polymerase is supplied with 3 vials of working solution:

10X PCR Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonidet P40;
10X PCR Buffer with (NH₄)2SO4: 750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM (NH₄)₂SO₄, 0.1% Tween 20;
25 mM MgCl₂ Solution