Components of pQY kit
| Cat No | Component | Promoter | Amount (µg) | Concentration (µg/µl) |
| P1-600-020 | pQYAN | ADH-1 | 10 | 0.5 |
| P1-601-020 | pQYAC | ADH-1 | 10 | 0.5 |
| P1-602-020 | pQYGN | GAL-1 | 10 | 0.5 |
| P1-603-020 | pQYGC | GAL-1 | 10 | 0.5 |
| A1-100-100 | Anti-E2Tag MAb (5E11) | 100 | 1.0 | |
| A1-900-050 | Anti-mouse IgG-HRP | 50 | 1.0 | |
| A1-901-050 | Anti-mouse IgG-AP | 50 | 1.0 | |
| A1-902-050 | Anti-mouse IgG-FITC | 50 | 1.0 |
Store kit at -20ºC RECOMMENDATIONS
· If the pQY vector does not meet expression requirements attempt cloning the gene of interest into the suitable pQ vector before subcloning it into the appropriate yeast vector.
· PCR cloning is recommended if the restriction enzyme sites do not fit with the cloning plan or precise cloning is necessary.
· For removal of the E2Tag, the protease Pro39 is available (Cat No Y2-100-100). Please note that neither the pQY nor the pQ vectors contain the Pro39 recognition site. The recognition sequence of Pro39 (-FDDVLRLGRAGA/YIFSSDTG-) can be introduced through the PCR cloning procedure.
