Yeast tagging and expression kit
(pQY kit) for E2Tag tagging, expression and detection of recombinant protein in yeast cells.Cat# K1-600-001
Vector contains either ADH-1 (high expression) or GAL-1 (inducible expression) promoter.
CLONING
- The gene of interest can be cloned into the suitable pQP vector using the restriction enzymes indicated in the MultiCloning Site (MCS), or through a PCR cloning approach.
- pQYAN and pQYGN vectors provide E2Tag epitope tagging options which allow for E2Tag tagging at the N-terminus of the protein.
- pQYAC and pQYGC vectors provide E2Tag epitope tagging options which allow for E2Tag tagging at the C-terminus of the protein.
- Recombinant protein expression in yeast is driven by GAL-1 (inducible promoter) or ADH-1 (strong promoter).
- pQY vectors contain the Leu2 selection marker.
- Easy immunotagging of recombinant proteins in yeast cells.
- Eliminates the need to create specific antibodies.
- The E2Tag tagged recombinant protein can be detected with E2Tag monoclonal antibody through immunoblotting or immunofluorescence analysis.
- Anti-E2Tag antibody is visualized with HRP, AP- or FITC-conjugated goat anti-mouse antibodies.
USER MANUAL: pQY kit (pdf)
RELATED PRODUCTS
R1-100-500 Anti-E2Tag affinity resin
Y2-102-005 Smart Taq Thermostable DNA Polymerase Set
Y1-100-005 Protease Pro39
Inquiries: sales [at] icosagen.com Other kits: mammalian expression, plant expressioin, bacterial cloning
