Plant tagging and expression kit
(pQP kit) for E2Tag tagging, expression and detection of recombinant proteins in plant cellsCat No K1-700-001
Vector contains CaMV 35S promoter and nos-terminator
CLONING
- The gene of interest can be cloned into the suitable pQP vector using the restriction enzymes indicated in MultiCloning Site (MCS), or by a PCR cloning approach.
- The pQP vectors provide E2Tag epitope tagging options which allow for E2Tag epitope tagging at either the N- or C-terminus of the protein.
- M13 and M13 reverse sequencing primer sequences enable convenient sequencing.
- Recombinant protein expression in plant cells is driven by the CaMV 35S promoter.
- Nos-terminator provides the signal required for termination of plant transcription.
- Easy immunotagging of recombinant proteins in plant cells.
- Eliminates the need to create specific antibodies.
- The E2Tag tagged recombinant protein can be detected with E2Tag monoclonal antibody through immunoblotting or immunohistochemical analysis.
- Anti-E2Tag antibody is visualized with HRP, AP- or FITC-conjugated goat anti-mouse antibodies.
USER MANUAL: pQP kit (pdf)
RELATED PRODUCTS
R1-100-500 Anti-E2Tag affinity resin
Y2-102-005 Smart Taq Thermostable DNA Polymerase Set
Y1-100-005 Protease Pro39
Inquiries: sales [at] quattromed.com Other kits: mammalian expression, yeast expression, bacterial cloning
