pQM kit

• The gene of interest can be cloned into the suitable pQM vector using the restriction enzymes indicated in the MultiCloning Site (MCS), or through a PCR cloning approach.
• The pQM vectors provide E2Tag epitope tagging options which allow for E2Tag epitope tagging to take place at either the N- or C-terminus of the protein.

• The human cytomegalovirus (CMV) immediate early promoter region produces a strong transient expression.
• The translational start sequence used in pQM-E2Tag-N vectors is the Kozak consensus sequence GCCATGG. 
• The pQM-E2Tag-N vector does not contain a stop-codon. 
• The pQM-E2Tag-C vector does not contain a translation start sequence. 
• The polyadenylation sequence provides the signal required for termination of both mammalian transcription and translation.

• Easy immunotagging of recombinant proteins. 
• Eliminates the need to create specific antibodies. 
• The E2Tag tagged recombinant protein can be detected with E2Tag monoclonal antibody through immunoblotting or immunofluorescence analysis. 
• Anti-E2Tag antibody is visualized with HRP, AP- or FITC-conjugated goat anti-mouse antibodies.