pQM-intron kit
for E2Tag tagging, expression and detection of recombinant proteins in mammalian cells.Cat No K1-210-001
CLONING
- The gene of interest can be cloned into the suitable pQM-intron vector using restriction enzymes indicated in the MultiCloning Site (MCS), or through a PCR cloning approach.
- The pQM-intron vectors provide E2Tag epitope tagging options which allow for E2Tag epitope tagging at either the N- or C-terminus of the protein.
- The human cytomegalovirus (CMV) immediate early promoter region produces a strong transient expression.
- A ß-globin intron located downstream from the enhancer/promoter region can further increase expression of recombinant protein.
- The translational start sequence used in pQM-E2Tag-N-intron vectors is the Kozak consensus sequence GCCATGG.
- The pQM-E2Tag-N-intron vector does not contain a stop-codon.
- The pQM-E2Tag-C-intron vector does not contain a translation start sequence.
- The polyadenylation sequence provides the signal required for termination of both mammalian transcription and translation.
- Easy immunotagging of recombinant proteins.
- Eliminates the need to create specific antibodies.
- The E2Tag tagged recombinant protein can be detected with E2Tag monoclonal antibody through immunoblotting or immunofluorescence analysis.
- Anti-E2Tag antibody is visualized with HRP, AP- or FITC-conjugated goat anti-mouse antibodies.
- PCR cloning is recommended if the restriction enzyme sites do not fit with the cloning plan or precise cloning is necessary.
- For removal of the E2Tag, the protease Pro39 is available (Cat No Y2-100-100). Please note that neither the pQM nor pQ vectors contain the Pro39 recognition site. The recognition sequence of Pro39 (FDDVLRLGRAGA/YIFSSDTG) can be introduced through the PCR cloning procedure.
USER MANUAL: pQM-intron kit (pdf)
RELATED PRODUCTS:
R1-100-500 Anti-E2Tag affinity resin
Y2-102-005 Smart Taq Thermostable DNA Polymerase Set
Y1-100-005 Protease39
