Intrac QP kit
for expression and detection of protein-protein interactions in mammalian cells.Cat No K1-220-001
CLONING
- The gene of interest can be cloned into the suitable pQM vector using the restriction enzymes indicated in the MultiCloning Site (MCS), or through a PCR cloning approach.
- The coding sequences of the desired proteins are cloned into the two expression vectors so that each are designated individually by the E2Tag or by the E4Tag.
- Following co-transfection of the plasmids, cellular expression of the tagged proteins can be observed.
- Vectors provide epitope tagging options which allow for E2Tag or E4Tag epitope tagging to take place at either the N- or C-terminus of the protein.
- The human cytomegalovirus (CMV) immediate early promoter region produces a strong transient expression.
- The translational start sequences used in the pQM-E2Tag-N and in the pQM-E4Tag-N are the respective Kozak consensus sequences GCCATGG and GCCACCATG.
- Neither the pQM-E2Tag-N nor the pQM-E4Tag-N vectors contain stop-codons.
- Neither the pQM-E2Tag-C nor the pQM-E4Tag-C vectors contain translation start sequences.
- The polyadenylation sequence provides the signal required for termination of both mammalian transcription and translation.
- Easy immunotagging of recombinant proteins.
- Eliminates the need to create specific antibodies.
- The anti-E2Tag affinity resin suspension allows for the capture and immunoprecipitation of the protein-protein complex of interest.
- Visualization of the co-immunoprecipitated protein can be achived with E4Tag antibody or specific antibody through immunoblot.
KIT COMPONENTS: 8 vectors and 4 sets of antibodies.
USER MANUAL: Intrac QP kit (pdf)
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