A3-708-100
Mouse
monoclonal antibody to Hev b5 (Major latex allergen)
Immunogen Description
Hev b5-MBP
fusion protein expressed and purified from E.
coli.
Alternative Names
Major latex allergen (Hevea brasiliensis)
Clonality
Mouse monoclonal
Reactivity
Recombinant and native Hev b5
Protocol
ELISA 1:64 000, WB. Reacts as detection antibody in capture
ELISA with Hev b5 binding monoclonal antibody A3-707-100
Purification
Protein G affinity purified
Buffer
PBS, 0.1% sodium azide
Related Products
A3-707-100 - mouse
monoclonal antibody to Hev b5, used as a binding antibody in pair with detection
antibody A3-708-100 in capture ELISA.
Monoclonal
antibodies to Hev b1, Hev b3 and Hev b6.02 are available. For more information
see www.icosagen.com/antibodies Shipping
This product is shipped in non-frozen liquid form in
ambient conditions
Storage
Store at - 20 …-70 °C upon receipt. Divide antibody
into aliquots prior usage. Avoid multiple freeze-thaw cycles as product
degradation may result
Background
Liquid latex from the rubber tree, Hevea brasiliensis,
is the source of natural rubber latex (NRL) and contains over 200 proteins; 14
of them have been identified as allergens. Only some allergens retain their
allergenic properties through the manufacturing processes. The NRL allergens
that have been shown to be clinically relevant to genuine NRL allergy, and
present in the final NRL products with maintained allergenicity are Hev b1, Hev
b3, Hev b5 and Hev b6.02.
Refrences
ASTM D7427 - 08e1
Standard Test Method for Immunological Measurement of Four Principal Allergenic
Proteins (Hev b 1, 3, 5 and 6.02) in Natural Rubber and Its Products Derived
from Latex.
Koh
D, Ng V, Leow YH, Goh CL. (2005) A study of natural rubber latex
allergens in gloves used by healthcare workers in Singapore. Br J Dermatol.
153(5):954-9.
Palosuo
T, Alenius H, Turjanmaa K. (2002) Quantitation of latex allergens.
Methods. 27(1):52-8.
Peixinho
C, Tavares-Ratado P, Tomás MR, Taborda-Barata L, Tomaz CT. (2008)
Latex allergy: new insights to explain different sensitization profiles in
different risk groups. Br J Dermatol. 159(1):132-6.
Kang PB, Vogt K, Gruninger SE, Marshall M, Siew C,
Meyer DM.
(2007) The immuno cross-reactivity of gutta percha points. Dent Mater.
23(3):380-4 Limitations
This
product is for research use only
