A2-405-100

Datasheet as PDF

Figure 1. Western-Blot analysis of HPV-11 E7 protein. WB was was carried out with 9H5 Mab using the lysates of U20S and Hela cells. Lines 1 and 4 are according cells transfected with plasmid expressing HPV-11  E7 protein, lines 2 and 3 non-transfected U2OS and Hela cells. Line 5. Protein size marker


Figure 2.  IP analysis of HPV-11 E7 protein.  Line 2 detection of E7 protein by Mab 9H5 in lysate of U20S cells. Line 3 the lower band is precipitated E7, upper bands are light and heavy chain of Mab. Line 4 is precipitation without mab 8G6 and Line 5. lane shows IP experiment with plain U2OS cells. Line 1. Protein size marker

Figure 3.  IF analysis of HPV-11 E7 protein in U2OS cells.IF was carried out with U2OS cells transfected with plasmid expressing HPV-11 E7 protein. All the nuclei were stained by DAPI.  Green color represents nucleus-localized HPV-11 E7 detected by Mab 9H5. IF analysis of E7 protein show similar result also in HeLa cells
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Immunogen
HPV-11 E7
Immunogen Description
Recombinant full-length HPV-11 E7-protein purified from E. coli
Uniprot ID
-
Alternative Names
-
Clonality
Mouse monoclonal
Clone
9H5
Class
IgG2a
Reactivity
Reacts with HPV-11 E7-protein.  Mapped to amino acids 36-70
Application
ELISA, WB, IF, IP
Protocol

ELISA: 1: 128 000, WB: 1: 1000, IP 1:500.

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification
purified with Protein G affinity chromatorgaphy
Buffer
PBS pH 7.4, with 0.1% sodium azide
Concentration
1 mg/ml
Unit Size
100 µg
Related Products
HPV-11 and HPV-18 E7 monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background
Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E7 proteins have cellular transforming and trans-activating activities
Refrences
-
Limitations
This product is for research use only